Human Fecal Sample Processing in Metagenomic Studies
The Wainamics sample preparation system more accurately identifies and quantifies species in a defined bacterial consortium than industry-standard stool metagneomic DNA extraction kit.
Client is developing a point-of-care gut microbiome profiling platform. They came to Wainamics for a fully automated sample preparation system for metagenomic DNA extraction.
For bench top, high sample number efficient testing, client required a fully automated sample prep system for cell lysis, DNA capture, and purification. System requirements included:
Disposable sample-prep cartridge
Intuitive and flexible user interface to allow for protocol optimization
Fast scalability to allow multiple experiment stations as company expands
Wainamics developed a fluidic cartridge incorporating filters and DNA extraction beads, together with the WP6 pump system in less than 4 weeks. Lysis, capture, and purification steps were incorporated with precise flow-rate and volume control. New protocols can be programmed for automated operation with the built-in recording feature.
Metagenomic DNA was successfully extracted using Wainamics sample preparation system. The resulting data demonstrated higher accuracy and less bias in extracting both Gm+ and Gm- bacteria from a defined sample than the industry-leading stool metagenomic DNA extraction kit.